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KMID : 0379520000160030179
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한국독성학회지
2000 Volume.16 No. 3 p.179 ~ p.185
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Zinc-induced Apoptosis in C6 glial Cells via Generation of Hydrogen Peroxide(H_2O_2)
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Lee Ji-enny
Kim Myung-Sunny
So Hong-Seob
Kim Nam-Song
Cho Kwang-Ho
Lee Hyang-Joo
Lee Ki-Nam
Park Kil-Rae
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Abstract
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Zinc is known to generate reactive oxygen species (ROS) including superoxide anion and hydrogen peroxide (H_2O_2), which eventually contribute to cytotoxicity in a variety of cell types. Here in, we demonstrated that zinc decreased the viability of C6 glial cells in a time and dose-dependent manner, which was revealed as apoptosis characterized by ladder-pattern fragmentation of genomic DNA. chromatin condensation and DNA fragmentation in Hoechst dye staining. Zinc-induced apoptosis of C6 glial cells was prevented by the addition of catalase and antioxidants including reduced glutathione (GSH), N-acetyl-L-cysteine (NAC) and pyrrolidinedithiocarbamate (PDTC). Wefurther confirmed that zinc decreased intrac-ellular levels of GSH and generated H_2O_2in C6 glial cells. Moreover, antioxidants also decreased the generation of zinc-induced H_2O_2 in C6 glial cells. These data indicated that zinc-induced the apoptotic death of C6 glial cells via generation of reactive oxygen species such as H_2O_2.
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KEYWORD
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Oxidative stress, Zinc, H_2O_2, C6 glial cells, Apoptosis
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